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. 2012 Dec 26;41(4):2340–2353. doi: 10.1093/nar/gks1338

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 4. — Delay in the de novo assembly of the plasmid partitioning complex in the clb5Δ, clb6Δ host strain. (A, B) The transient dissociation and reassociation of Rep1 at STB in cells released from G1 arrest was assayed by ChIP in wild-type and clb5Δ, clb6Δ strains. Gross DNA replication was followed by fluorescence-activated cell sorting analysis. (C, D) Association times of Rsc8 with STB after release from G1 were monitored by ChIP in wild-type and clb5Δ, clb6Δ strains. (E) Replication times of STB were followed by ChIP using an antibody against BrdU. In the plot for BrdU incorporation, the band intensities of the PCR amplified DNA from an assay were normalized to the highest band intensity from that assay, which was assigned a value of 100%. The experiments were conducted using strains K699 to MJY5048 listed in Supplementary Table S1.