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. 2012 Dec 26;41(4):2581–2593. doi: 10.1093/nar/gks1353

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — ADAR2 colocalizes with SFRS9 and RPS14. Cells stably transfected with FLAG-tagged rat ADAR2 were cotransfected with an empty vector or myc-tagged DDX15, SFRS9 or RPS14. The green FITC channel shows the localization of ADAR2, while the candidate proteins are shown in the red TRITC channel. Immunofluorescence staining indicates that SFRS9 and RPS14 colocalize with ADAR2 to nucleoli, whereas DDX15 localizes in nuclear dots that do not overlap with nucleoli. In normal cells that do not overexpress ADAR2, SFRS9 is localized to the nucleoplasm (see Supplementary Figure S7). The colocalization of SFRS9 to nucleoli therefore depends on the presence of ADAR2. The colocalization also reflects the coimmunoprecipitation of ADAR2 with RPS14 and SFRS9 shown in Figure 7.