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. 2013 Jan 7;41(4):2644–2658. doi: 10.1093/nar/gks1453

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Effects of AUF1 and AGO2 abundance on mRNA binding. K562 cells were transiently transfected with plasmids encoding control shRNA or shRNA against either AUF1 or AGO2. Two days after transfection of shRNA-expressing plasmids, lysates were prepared and fractionated by mRNP-immunoprecipitation with control antibodies or anti-AUF1 antibody (for AGO2-knockdown cells) or anti-AGO2 antibody (for AUF1-knockdown cells). Both input and immunoprecipitated materials were analysed by qRT–PCR, and abundances of the indicated mRNAs were plotted as a percent of the input mRNA level. The means ± SD from three independent experiments are shown. **P < 0.01 versus control shRNA. (A) Knockdown of AUF1 followed by AGO2 mRNP-immunoprecipitation. (B) Knockdown of AGO2 followed by AUF1 mRNP-immunoprecipitation.