. 2013 Jan 7;41(4):2644–2658. doi: 10.1093/nar/gks1453

Table 2.

Effects of AUF1 and/or AGO2 knockdown on endogenous mRNA decay kinetics in K562 cells

mRNA	Control shRNA		AUF1 shRNA		AGO2 shRNA		AUF1 + AGO2 shRNA
	k (h⁻¹)^a	t₁_/₂ (h)^b	k (h⁻¹)^a	t₁_/₂ (h)^b	k (h⁻¹)^a	t₁_/₂ (h)^b	k (h⁻¹)^a	t₁_/₂ (h)^b
MYC	1.46 ± 0.04	0.48	1.33 ± 0.04	0.52	0.90 ± 0.07**	0.77	1.30 ± 0.03	0.53
IFI16	0.23 ± 0.01	3.0	0.09 ± 0.01^*	8	0.05 ± 0.01**	>10	0.08 ± 0.01^*	8.7
IL1RN	0.26 ± 0.01	2.7	0.05 ± 0.01**	>10	0.34 ± 0.03	2.0	0.07 ± 0.01^*	10
FBXO24	0.16 ± 0.01	4.3	0.04 ± 0.01**	>10	0.03 ± 0.01**	>10	0.06 ± 0.01^*	>10
HOXB8	0.20 ± 0.01	3.5	0.12 ± 0.01^*	5.8	0.03 ± 0.01^*	>10	0.05 ± 0.01^*	>10
THRAP5	0.09 ± 0.01	8	0.09 ± 0.01	8	0.12 ± 0.01	5.8	0.06 ± 0.01	>10
NDOR1	0.07 ± 0.01	10	0.16 ± 0.01^*	4.3	0.06 ± 0.01	>10	0.07 ± 0.01	10
GNLY	0.09 ± 0.01	8	0.07 ± 0.01	10	0.09 ± 0.01	8	0.08 ± 0.01	9
DOHH	0.51 ± 0.02	1.4	0.12 ± 0.01^*	5.8	0.20 ± 0.01^*	3.5	0.14 ± 0.01^*	5.0

^aFirst-order decay constants derived from plots of percent mRNA remaining versus time following actinomycin D treatment, as described in ‘Materials and Methods’ section. Values represent the means ± SD for n ≥ 3 independent experiments.

^bAverage mRNA half-lives were calculated as t₁_/₂ = ln2/k.

*P < 0.05; **P < 0.01 versus control shRNA.