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. 2012 Dec 26;41(4):2594–2608. doi: 10.1093/nar/gks1361

Figure 9.

Figure 9.

S3L2-ACUucc SARS-CoV mutant RNA synthesis and protein expression kinetics. Cultures of VeroE6 cells were infected with wild-type or S3L2-ACUucc mutant SARS-CoV at an MOI of 1 or mock-infected. Representative northern blots with sgRNA and gRNA species and molecular weights indicated by arrows. (a) probed total RNA to visualize genomic and subgenomic viral RNA and (c) probed polyA enriched RNA to visualize the sgRNA species. Delays in RNA synthesis kinetics in S3L2-ACUucc frameshift mutant. Bars represent the densitometry measurements of fold change over mock for mock-infected, wild-type and S3L2-ACUucc SARS-CoV mutant RNA at the indicated times pi. (b) Densitometry of gRNA. (d) Densitometry of sgRNA. ORF1a versus ORF1b protein expression kinetics in S3L2-ACUucc frameshift mutant. Proteins were separated on polyacrylamide gels and probed with rabbit sera directed against (e) the replicase proteins nsp1 or (f) nsp16 as indicated. Size markers are indicated to the left of each blot.