Figure 3.

Individual D-Ser variants of the αA-crystallin peptide, TVLDSGISEVR as a function of age, in normal and cataract lens proteins. In each case, the peak intensities were expressed as a percentage of the total ion abundance for TVLDSGISEVR (symbols: □ Normal ♦ Cataract ). (a) D-Ser (59)/ L-Ser (62) (normal; y = 0.103x + 0.576, R² = 0.987, two-sided P = 0.0007). Mann–Whitney U test, two-sided P = 0.743. (b) L-Ser (59)/ D-Ser (62) (normal; y = 0.29x ( 1.27, , R² = 0.918, two-sided P = 0.0103). Mann–Whitney U test, two-sided P = 0.886. (c) D-Ser (59)/D-Ser (62) (normal; y = 0.0495x ( 0.218, R² = 0.96, two–sided P = 0.0034). Mann–Whitney U test, two-sided P = 0.0286. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]