Fig. 2.

Effect of acute permeability barrier disruption on the inside-out barrier function of TJ in murine epidermis. Skin samples were taken from untreated skin and barrier-disrupted skin at 30 min and 1 h after tape-stripping. Freshly obtained skin biopsies were submerged en bloc in 4% colloidal lanthanum nitrate and post-fixed in osmium tetroxide and examined by transmission electron microscope. Insets show the enlarged view of upper stratum granulosum (SG) and stratum corneum (SC) layer. Bars: 2 µm. TJ, tight junction.