FIG. 1.

TCDD modulates the expression of genes critical to the formation of the cornified layer. (A) Depiction of the microarray analysis of the EDC, located on human chromosome 1q21, following treatment with TCDD. The level of RNA corresponding to the genes in bold print (normal and italics) had increased expression in response to TCDD (24h). The genes in italic bold print confirmed previously published increases in expression (Sutter et al., 2011). The RNAs of the remaining genes were not significantly altered by TCDD. (B)–(D) qRT-PCR was used to verify the FC values obtained in microarray analysis for the indicated genes following TCDD treatment (24h). Levels of RNA (mean [n = 4] ± SD) are expressed in units relative to the DMSO control, given a value of 1. *p < 0.05 indicates a significant difference between the control and treated samples.