FIG. 4.

CAR RE of 97-bp within Kcnk1 promoter.

(A and C) Reporter activities of Kcnk1 promoter were determined in HNF4-positive HepG2 and HNF4-negative COS1 cells transfected with or without CAR and HNF4α, respectively. Values are expressed as the relative intensity of Firefly luciferase activity (Kcnk1 promoter) normalized to the intensity of Renilla luciferase activity (internal control). Data are mean ± SD (n = 3 in each group). (B) The core sequences of DR4 site and DR1 sites within the 97-bp CAR RE (97-bp RE) are shown.