FIG. 6.

Hepatic hyperplasia in the livers of phenobarbital-treated Kcnk1 ^−/− mice.

(A and B) Liver sections were prepared from mouse livers as described in Materials and Methods section. The incorporation of BrdU within the liver lobule was determined by immunohistochemistry. (A) Data shown are representative photographs from section slides of five individual mice (original magnification ×200). Z1, Z3, and PB denote zone 1, zone 3, and phenobarbital, respectively. Scale bar = 100 µm. (B) BrdU-positive hepatocytes were counted. Values are shown as a percentage of BrdU-positive hepatocytes over total nuclei. Data are mean ± SD (n = 5 in each group). *p < 0.05, **p < 0.01, ***p < 0.005 for PBS-treated mice vs. PB-treated mice, ^† p < 0.05, ^††† p < 0.005 for Kcnk1 ^+/+ vs. Kcnk1 ^−/− mice, Tukey’s multiple comparison test. PB = phenobarbital.