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. Author manuscript; available in PMC: 2013 Feb 19.
Published in final edited form as: Insect Biochem Mol Biol. 2011 May 27;41(9):707–714. doi: 10.1016/j.ibmb.2011.05.002

Figure 4.

Figure 4

Activity assay using tryptamine as a substrate. aaNAT1 and aaNAT2 were tested using tryptamine as the acetyl group acceptor and Acetyl-CoA as acetyl group donor using method 2 described in the Method section. N-acetyltryptamine was detected in the reaction mixture. X-axis represents elution time (min) and Y-axis indicates electric current generated during oxidation of tryptamine and N-acetyltryptamine at the working electrode. In chromatograms, arrow indicates time of sample application. Chromatograms illustrate the production of N-acetyltryptamine in a tryptamine and Acetyl-CoA mixture in the presence of aaNAT1 (A), the production of N-acetyltryptamine in a tryptamine and Acetyl-CoA mixture in the presence of aaNAT2 (B), and the retention time of tryptophan and N-acetyltryptamine standards in the absence of aaNAT1 or aaNAT2 under identical analysis conditions by HPLC with electrochemical detection (C).