. Author manuscript; available in PMC: 2013 Feb 19.

Published in final edited form as: Insect Biochem Mol Biol. 2011 May 27;41(9):707–714. doi: 10.1016/j.ibmb.2011.05.002

Table 4.

Kinetic analysis of aaNAT1 and aaNAT2. The activities were measured as described in the Materials and Methods section. The parameters were calculated by fitting the Michaelis–Menten equation to the experimental data using the enzyme kinetics module. Results are means ± SE.

	K_m mM	k_cat min^-1	k_cat/K_m min^-1mM^-
aaNAT1
octopamine	0.14±0.08	435.5±67.0	3111
norepinephrine	0.16±0.04	465.5±34.6	2909
tryptamine	0.16±0.04	440.7±31.7	2754
methoxy-tryptamine	0.18±0.06	461.6±44.8	2564
tyramine	0.17±0.04	406.5±24.8	2391
dopamine	0.19±0.07	438.0±44.5	2305
serotonin	0.23±0.06	452.7±35.7	1968

aaNAT2
dopamine	0.13±0.05	153.5±15	1181
tyramine	0.17±0.03	190.1±11	1118
tryptamine	0.17±0.04	183±11.8	1076
methoxy-tryptamine	0.23±0.04	224.4±11	976
serotonin	0.23±0.05	189.7±11.9	825
octopamine	0.27±0.04	218.8±10.5	810
norepinephrine	0.42±0.06	208.2±11.5	496