AGC1, COL1A1, COL2A1, LEP, SPP1, SOX9, and TUBB3 (corrected with the housekeeping gene, 18s) gene expression results at the end of the 7 day 3-D agarose culture. Relative Quantitation (RQ) to day 0 control cells (also corrected with the housekeeping gene, 18s) from the same monolayer expansion conditions. n≥ 4 constructs per condition. Values are the mean ± SEM. ** = P < 0.0001; * = P < 0.05; ## = P < 0.0001 relative to all other conditions and # = P < 0.05 relative to all other conditions unless otherwise noted. SEHI: Suspension culture plastic, Expansion medium, High density; SELO: Suspension culture plastic, Expansion medium, Low density; TEHI: Tissue culture plastic, Expansion medium, High density; TSHI: Tissue culture plastic, Stromal medium, High density; TSLO: Tissue culture plastic, Stromal medium, Low density