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. Author manuscript; available in PMC: 2013 Feb 19.
Published in final edited form as: J Neuroimmunol. 2011 Jul 1;237(1-2):47–56. doi: 10.1016/j.jneuroim.2011.06.002

Figure 7.

Figure 7

Effect of antagonist treatment and context on LPS-induced expression of IL-6 mRNA in the spleen as determined by real-time RT-PCR (top panel) and IL-6 protein in the spleen as determined by ELISA (bottom panel). Exposure to the previously heroin-paired context (Saline, white bar) resulted in a conditioned suppression of IL-6 mRNA and protein levels in the spleen as compared to the home cage control group (Saline, black bar). Disconnection of the VTA-BLA-NAC circuit blocked the effect of the conditioned stimulus (Contralateral, white bar). Ipsilateral administration of intra-NAC AP5 and intra-BLA SCH23390 did not alter the conditioned suppression of IL-6 (Ipsilateral, white bar). Real-time RT-PCR data are expressed as IL-6 copy number per 10 ng cDNA based on a standard curve using Roche LightCycler software. ELISA data are expressed as pg of IL-6 protein per mg of total protein.