FIGURE 10.

Effect of C18- and C20-ceramide on ST2 adipogenesis. A, ST2 cells were treated with 20 μm C18-ceramide (C18-cer), or 15 μm C20-ceramide (C20-cer) during adipogenesis. The cells were stained with Oil Red O on day 6 of differentiation. Scale bar, 100 μm. B, the Oil Red O, which accumulated in lipid droplets, was extracted with isopropyl alcohol and measured at OD 535 nm. C, ST2 cells were treated with 20 or 25 μm C18-ceramide and 7 μm or 15 μm C20-ceramide during adipogenesis. The relative triglyceride levels were measured on day 6 of differentiation and normalized to the total protein levels. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars, S.D.