FIGURE 2.

Expression profiling of Fam57b in ST2 adipogenesis and mouse different tissues. A, samples were harvested on the indicated days after adipogenic induction, and total RNA was extracted and analyzed by real-time RT-PCR with primers targeting a common sequence (indicated by Fam57b_c2) and variant-specific sequences (indicated by Fam57b_var1, -2, and -3). As a control, samples left uninduced were also analyzed by qRT-PCR using the common sequence primers (Fam57b_c2(C)). B, 3T3L1 cells were differentiated into adipocytes, and samples were harvested on the indicated days. Total RNA was extracted and analyzed for Fam57b expression using common primers (Fam57b_c2) as well as specific primers for variants 1 and 2 (Fam57b_var1 and Fam57b_var2). C, ST2 cells were induced with rosiglitazone instead of a total adipogenic mixture and analyzed on the indicated days of differentiation. The mRNA expression of three Fam57b variants was analyzed by real-time RT-PCR as indicated above. D, the expression of the Fam57b variants in normal tissues from C57bBL/6J mice was analyzed by real-time RT-PCR with variant-specific primers. BAT, brown adipose tissue; WAT, white adipose tissue; AdreG, adrenal gland; CortB, cortical bone; Calv, calvaria; BM, bone marrow. E, the expression of the Fam57b variants was examined in adipose tissue derived from obese mice induced by a high fat diet (HFD), and from mice fed a normal diet (ND). Two different primers were used for variants 1 and 2 (a and b). Results are the means ± S.D. (error bars) (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001.