FIGURE 6.

Adipogenic differentiation is promoted by Fam57b down-regulation in ST2 cells. A, ST2 cells were transfected with three classes of Fam57b siRNA (siFam57b-A, -B, and -C) targeting a common site in all of the Fam57b variants. Non-targeting control siRNA (siNC) and Pparγ siRNA (siPparγ) were used as a negative and positive control, respectively. At 6 h post-transfection, adipogenesis was induced, and total RNA was extracted from the cells on day 2. The knockdown efficiency on day 2 of differentiation was analyzed by real-time RT-PCR. B, ST2 cells were treated with siRNA and adipogenic induction and then fixed and stained with Oil Red O on day 6. Magnification was ×10. Scale bar, indicates 100 μm. C, the triglyceride (TG) content was analyzed on day 6 of differentiation. D, markers of adipocyte differentiation were analyzed on day 6 of differentiation by qRT-PCR. Pparγ, AdipoQ and Glut4 were used as mature adipocyte markers, and perilipin 1 (Plin1) was used as a lipid droplet marker. Results are means ± S.D. (error bars) (n = 3); *, p < 0.05; **, p < 0.01; ***, p < 0.001.