FIGURE 3.

Lyso-PS drives engulfment of viable exudate neutrophils (neut) by macrophages in vivo. Exudate neutrophils (5 × 10⁶) from WT and gp91^phox−/− mice, with or without lyso-PS liposome premixing (see “Experimental Procedures”), were adoptively transferred into the peritonea of WT or G2A^−/−-recipient mice on day 5 following induction of peritonitis. After 4 h, peritoneal cells were collected by lavage, and the phagocytic index was determined by visual inspection of cytospins. A and B, n = 5; *, p < 0.05 compared with WT neutrophil control. C–E, lavaged cells were TUNEL-stained and analyzed for free TUNEL⁺ neutrophils (C) and phagocytic index of MΦ containing TUNEL⁺ material in phagosomes (D). n = 5; *, p < 0.05 compared with WT neutrophil control. E, representative photographs of cytospins from C and D. Red arrows indicate TUNEL⁺ material in phagosomes. The dotted line in A, B, and D represents the background phagocytic index from control mice that did not receive adoptive transfer of exudate neutrophils. Data represent mean ± S.E.