FIGURE 6.

Adoptive transfer of lyso-PS^high exudate neutrophils (neut) enhances macrophage CD206^high expression during peritonitis. A, lyso-PS^high (WT) exudate neutrophils (1 × 10⁷) (or PBS) were adoptively transferred into the peritonea of WT recipient mice at 12 h into zymosan-induced peritonitis. Peritoneal cells were collected by lavage 24 h later, and CD206 expression on F4/80+/Ly6G- MΦ was determined by flow cytometry and expressed as in Fig. 5. Left, histograms from a single experiment. Staining is shown with anti-CD206 (solid line) or CD206 isotype control antibody (shaded gray). Right, summated expression data. n = 8. B, left, time course of total neutrophils and macrophages (Mac) following induction of zymosan peritonitis in gp91^phox−/− mice. Right, time course of CD206 expression on macrophages in WT or gp91^phox−/− mice following induction of zymosan peritonitis. n = 8–10; *, p < 0.001 compared with gp91^phox−/−. C, representative histograms from WT PBS control or gp91^phox−/− recipients. Lower left, summated CD206 expression data are shown for gp91^phox−/− recipient MΦ. Dashed line represents CD206 expression on MΦ from PBS- treated WT mice. n = 8; *, p < 0.0001 compared with WT PBS control; **, p < 0.0002 compared with CD206 expression on PBS treated gp91^phox−/− mice. Data represent mean ± S.E.