FIGURE 7.

PGC-1β inhibits PDGF-induced MCM4 expression and MCM complex chromatin loading in VSMCs. A–F, VSMCs were infected with AdPGC-1β, AdGFP, AdshPGC-1β, or AdshLacZ, maintained in 0.2% FBS medium for 24 h, and then stimulated with 10 ng/ml PDGF-BB for 8 h or 18 h. Cyclin D1 (Ccnd1), MCM4, and MCM2 mRNA levels were analyzed by qRT-PCR. 18 S RNA served as internal control. n = 3. G and H, VSMCs were infected with AdPGC-1β, AdGFP, AdshPGC-1β, or AdshLacZ, maintained in 0.2% FBS medium for 24 h, and then stimulated with 10 ng/ml PDGF-BB for 12 h. VSMCs were collected and separated into total cell lysates or chromatin-bound extracts. Immunoblotting with the indicated antibodies was performed on lysates from equal cell numbers loaded onto each lane. *, p < 0.05; **, p < 0.01 versus AdGFP-treated group; #, p < 0.05; ##, p < 0.01 versus AdshLacZ-treated group.