Skip to main content
. 2012 Dec 19;288(7):4637–4648. doi: 10.1074/jbc.M112.369058

FIGURE 4.

FIGURE 4.

Persistence of binding of autophosphorylated CaMKII to CaV2.1(1848–1964). A, binding of CaMKII was detected by GST pulldown assay. CaV2.1(1848–1964) was incubated with CaMKII (20 nm) and Mg2+(5 mm) in the presence/absence of Ca/CaM (5 μm) and ATP (1 mm) as indicated. Samples were washed in the absence or presence of 5 mm EGTA as indicated. a, the blot was probed with anti-CaMKII antibody. b, the same blot after stripping and re-probing using anti-phospho-CaMKII(Thr-286). c, the same blot after stripping and reprobing using anti-CaM. d, the same blot after stripping and re-probing using anti-GST to show equal loading of CaV2.1(1848–1964). B, quantitation of relative CaMKII binding using anti-CaMKII under the indicated conditions (mean ± S.E.; **, p < 0.01; ***, p < 0.001 by Student's t test; n = 4).