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. 2012 Dec 19;288(7):4637–4648. doi: 10.1074/jbc.M112.369058

FIGURE 5.

FIGURE 5.

Persistence of binding of CaMKII after dephosphorylation. Purified recombinant protein phosphatase 1 (PP1) was added before (Pre-PP1) or after (Post-PP1) the kinase-channel complex was established as indicated. Washes were carried out in the presence or absence of 5 mm EGTA as indicated. A, binding of CaMKII to CaV2.1(1848–1964) GST was detected by GST pulldown assay. a, the blot was probed with anti-CaMKII. b, the same blot is shown after stripping and re-probing using anti-phospho-CaMKII(Thr-286). c, the same blot after stripping and re-probing using anti-GST to show equal loading of CaV2.1(1848–1964). B, binding of CaMKII to GST alone (control) was detected by GST pulldown assay. a, the blot was probed with anti-CaMKII. b, the same blot after stripping and re-probing using anti-phospho-CaMKII(Thr-286). c, the same blot after stripping and re-probing using anti-GST to show equal loading of GST.