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Schematic representation illustrating the strategy on CAP surface with the capture of the biotinylated ligand [Δ17]Arf1-GDP (Arf1, red) on the captured streptavidin conjugated with a ssDNA oligo (green), the saturation with biotin of free streptavidin sites (biotin, blue circle), and subsequent injection of the Arno analyte sample (Arno, purple) and complete regeneration of the CAP surface. The binding of Arno to [Δ17]Arf1 is assumed to be concomitant to the release of GDP.
