TABLE 7.

Kinetic constants of [Δ17]Arf1-Mg²⁺-GDP binding to captured Arno4M and Arno in the presence of 100 μm BFA with 2 μm GDP during association phase, derived from data displayed in Fig 6, panels A and B

SR = stoichiometric ratio.

Langmuir1:1 reversible binding model
Arno4M	ka 6.27 103 1/m·s	kd 3.26 10−4 1/s	Rmax 192 RU	kd/ka 52 nm	χ2 0.81	SR 0.79
Heterogenous ligand binding model
Arno	ka1 4.62 103 1/m·s	kd1 1.59 10−3 1/s	Rmax 1 49 RU	kd1/ka1 344 nm	χ2 0.96	SR 1 0.19
	ka2 3.32 104 1/m·s	kd2 3.61 10−2 1/s	Rmax 2 51 RU	kd2/ka2 1086 nm		SR 2 0.19