FIGURE 2.

Proteasome degradation pathway is involved in high glucose-induced AMPKα2 degradation. A, C2C12 cells were pre-treated 5 μm MG132 for 1 h and stimulated with 30 mm glucose incubation for 48 h. The cell lysates (20 μg) were analyzed via Western blotting for anti-AMPKα2 antibody. The anti-β-actin antibody was a protein loading control. B, C2C12 cells were pre-treated 5 μm lactacystin for 1 h and incubated with 30 mm glucose for 48 h. The cell lysates (20 μg) were analyzed via Western blotting for anti-AMPKα2 antibody. The anti-β-actin antibody was a protein loading control. C, C2C12 cells were immunoprecipitated with anti-AMPKα2 antibody. The immunoprecipitates were analyzed via Western blotting for anti-ubiquitin and AMPKα2 antibodies. TCL, total cell lysates.