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. 2013 Jan 4;288(7):4673–4680. doi: 10.1074/jbc.M112.406009

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — AMPKα2 interacts with WWP1. A, HEK293 cells were transiently transfected with Flag-AMPKα2 for 48 h and immunoprecipitated with anti-Flag antibody. The immunoprecipitates were analyzed via Western blotting for anti-WWP1 and Flag antibodies. B, cell lysates (1 mg) of C2C12 cells were immunoprecipitated with anti-AMPKα2 antibody. The immunoprecipitates were analyzed via Western blotting for anti-WWP1 and AMPKα2 antibodies. C, HEK293 cells were transiently transfected with Flag-WWP1 for 48 h and immunoprecipitated with anti-Flag antibody. The immunoprecipitates were analyzed via Western blotting for anti-AMPKα2 and Flag antibodies. TCL, total cell lysates.