FIGURE 7.

Expression of WWP1 blocked metformin-induced glucose uptake. A, L6 myoblast cells were differentiated for 7 days. Cells were transiently transfected with Flag-WWP1 for 48 h and then treated with metformin for 16 h. Glucose uptake was measured using 2-deoxy-d-[³H]glucose. *, p < 0.05 versus basal. B, L6 myoblast cells were differentiated for 7 days. Cells were incubated to 30 mm glucose for 48 h in the presence of or absence of metformin for 16 h. Glucose uptake was measured using 2-deoxy-d-[³H]glucose. *, p < 0.05 versus high glucose.