FIGURE 2.

Time courses of PrP^a and PrP^b fibril formation in the presence of homologous and heterologous seeds. Monomeric PrP (22 μm) was seeded with 0.5% (v/v) of fibrils, and the kinetics were followed by ThT fluorescence. A, monomeric PrP^a (empty circles) and PrP^b (filled circles) were aggregated in the presence of preformed fibrils of homologous PrP (PrP^a with PrP^a fibrils; PrP^b with PrP^b fibrils). B, monomeric PrP^a (empty circles) and PrP^b (filled circles) were aggregated in the presence of preformed fibrils of heterologous PrP (PrP^a with PrP^b fibrils; PrP^b with PrP^a fibrils). C, the lag phases for PrP^a and PrP^b in the presence of homologous (Homo.) and heterologous (Hete.) seeds were calculated as described under ”Experimental Procedures.“ The average of three replicates was plotted for each reaction. Error bars indicate ± S.E.