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PrPb prefibrillar (ThT-negative) oligomer formation monitored by p-FTAA. Monomeric PrPb (20 μm) was put into aggregation assays in 1.5-ml test tubes. 50 μl was withdrawn at different times and incubated with p-FTAA (squares) or ThT (circles) to monitor the formation of prefibrillar oligomers and fibril formation, respectively. The assay was carried out in the absence (filled symbols) or in the presence (empty symbols) of 1% (v/v) of seeds.
