FIGURE 6.

Conformational stability assays of PrP fibrils formed under seeded and unseeded conditions. A–F, Western blot of PrP^a (A–C) and PrP^b (D–F) fibrils formed in the absence (A and D) or in the presence of homologous (B and E) or heterologous (C and F) seeds and subjected to GdnHCl-induced denaturation and proteinase K digestion. 12 μl of proteinase K-treated samples was analyzed by Western blotting using SAF83 antibody, as described under “Experimental Procedures.” MW, molecular weight markers.