Cross-linking of Plugin by AgTG3.
A, Coomassie staining and B, fluorescence for SDS-PAGE of cross-linking reactions in presence of FITC-CAD. Lanes: 1, AgTG3 and Ca2+; 2, Plugin and Ca2+; 3, AgTG3 and Plugin-C without Ca2+; 4, AgTG3, Plugin-C, and Ca2+; 5, AgTG3, Plugin-C, Ca2+, and EGTA; 6, AgTG3, Plugin-C, Ca2+, and GTP.