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Specific activity of AgTG3. A, conjugation of FITC-CAD to Plugin-C in solution by AgTG3 (9.6 μg/ml, 30 °C). B, HRP-streptavidin assay (TMB, A405) for conjugation of biotin-TQQVEL to polylysine plates by 1.5 units mg−1 guinea pig TG2 (□) or AgTG3 (○). C, fluorescence (I510) for conjugation of FITC-CAD to Plugin-coated plates by 1.5 units mg−1 guinea pig TG2 (□) or AgTG3 (○). D, inhibition of AgTG3 by iodoacetamide (IA) versus negative control (NC) in Plugin/FITC-CAD assay.
