FIGURE 5.

Effect of αE-catenin or vinculin depletion on E-cadherin-mediated cell adhesion strength. A, Western blot analysis on lysates of Ecad cells treated for 72 h with 12 nm ctnna1 (αE-catenin) siRNA or non-targeting Ctrl smart pool siRNA. B, flow cytometry analysis of surface levels of E-cadherin in ctnna1 or Ctrl siRNA-treated cells. C, Western blot analysis of Ecad cell lysates after the cells were treated for 72 h with 16 nm of Vcl siRNA or Ctrl siRNA. D, flow cytometry analysis of surface levels of E-cadherin in Vcl (dark gray) or Ctrl (light gray) siRNA-treated cells. E, mean SF measured at 4 min of contact for Vcl siRNA-treated (dark gray bar) or Ctrl siRNA-treated Ecad cells (light gray bar). α-tub, α-tubulin. Error bars, S.E.