FIGURE 6.

Detection of vinculin in Ecad cells expressing the modulation domain of αE-catenin. A, GFP signals and immunolocalization of vinculin in Ecad cells transfected to express GFP alone or aa491–660 αEcatGFP mutant corresponding to the modulation domain. Arrows, cell-cell contacts between transfected Ecad cells (GFP⁺) and their neighbors. B, a representative plot profile showing the fluorescence intensity (expressed in arbitrary units (AU)) of vinculin along a line perpendicular to the cell-cell contact (corresponding to the cell-cell contact marked by an asterisk in the bottom panels in A) for Ecad cells expressing GFP (black line) or aa491–660 αEcatGFP mutant (gray line). C, Western blot analysis of lysates of Ecad cells transfected to express GFP or aa491–660 αEcatGFP mutant with anti-vinculin and α-tubulin antibodies. Error bars, S.E.