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. 2012 Dec 26;288(7):5027–5038. doi: 10.1074/jbc.M112.411173

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FIGURE 3. — Sall4 interacts with Oct-3/4. A, IP-IB assays of endogenous Sall4 and Oct-3/4 in mouse ES cell extracts are shown. Proteins were immunoprecipitated and detected by immunoblotting using indicated antibodies. B, IP-IB assays of FLAG-Sall4 and Oct-3/4-His are shown. FLAG-tagged Sall4 and Oct-3/4-His were transiently expressed in COS-7 cells as indicated, and IP-IB assays were performed. C, shown is a GST pulldown assay. In vitro translated and ³⁵S-labeled Oct-3/4 was analyzed as in Fig. 1C.