Sall4 co-occupies the Oct-Sox elements concurrently in combination with Oct-3/4 or Sox2.
A, shown are the Oct-Sox elements of the indicated genes. Nucleotide sequences of Oct-Sox elements reported with the seven indicated loci are shown (5–7, 10–14). Spacer refers to a sequence intervening between the Oct-3/4 binding and Sox2 binding sequences. B, ChIP analyses show that Sall4, Sox2, and Oct-3/4 occupy the Oct-Sox elements. Control 1 is within the first intron of Pou5f1, and control 2 is within the Atbf1 promoter. The error bars show S.D. OS shows the Oct-Sox element. p values are based on a one-tailed Student's t test (n = 3). C, sequential ChIP (Re-ChIP) analyses showed that Sall4 and Sox2 as well as Sall4 and Oct-3/4 simultaneously occupy the Oct-Sox elements. The error bars show S.D. p values are based on a one-tailed Student's t test (n = 3).