FIGURE 1.

Differential expression of the mitochondrial GSH transporters, OGC and DIC, within CGNs and cerebellar astrocytes. A, primary rat CGN cultures were fixed and stained for β-tubulin (red), MAP2 (green), and Hoechst (blue). Primary rat astrocyte cultures were fixed and stained for GFAP (green) and Hoechst (blue). B, CGN and cerebellar astrocyte cultures were subfractionated into mitochondrial and cytoplasmic fractions using differential centrifugation as described under “Experimental Procedures.” Protein lysates from the mitochondrial and cytosolic fractions were resolved using SDS-PAGE and immunoblotted for DIC and OGC. Immunoblotting for β-tubulin was used as a measure of fraction purity.