FIGURE 6.

Inhibition of DIC-dependent mitochondrial GSH transport does not render CGNs more susceptible to apoptosis induced by deprivation of depolarizing potassium. A, CGN cultures were treated with vehicle (Con) or 5 mm butylmalonate (BM) overnight. The following day, medium was either changed to 25 mm potassium minus serum (Con or BM) or changed to 5 mm potassium minus serum (5K or BM + 5K). Cells were then fixed, and nuclei (Hoechst; blue) were stained. Panels show decolorized nuclei from representative fields. Scale bar, 10 μm. B, cells were treated as in A, after which cells were fixed, and nuclei were stained with Hoechst. Apoptosis was quantified by determining the percentage of cells with condensed and/or fragmented nuclei. ##, p < 0.01 versus butylmalonate alone; **, p < 0.01 versus control; NS, not significant; n = 3 experiments with each treatment performed in duplicate. Error bars, S.E.