FIGURE 7.

A cell-permeable form of GSH protects CGNs from the sensitizing effects induced by inhibition of DIC-dependent mitochondrial GSH transport. A, CGN cultures were treated with vehicle (Con) or 5 mm butylmalonate (BM) overnight. The following day, cells were either left untreated (Con or BM) or treated with 12.5 μm HA14-1 in the presence or absence of 2 mm GSH monoethylester (GSHMEE) for 4 h, after which cells were fixed, and nuclei (Hoechst; blue) were stained. The left panels show decolorized nuclei from representative fields. The right panels show corresponding bright field images. Scale bar, 10 μm. B, cells were treated as in A; in addition, some cells were incubated with 25 μm SNP in the presence or absence of 2 mm GSH monoethylester. Cells were fixed, and nuclei were stained with Hoechst. Apoptosis was quantified by determining the percentage of cells with condensed and/or fragmented nuclei. **, p < 0.01 versus butylmalonate alone; +, p < 0.05 versus butylmalonate + SNP; ##, p < 0.01 versus butylmalonate + HA14-1; n = 3 experiments with each treatment performed in duplicate. Error bars, S.E.