FIGURE 4.

CideB interacts with COPII components. Rat hepatic cytosol (150 μg of protein) was incubated with anti-goat CideB antibody bound to agarose beads at 4 °C overnight. Immunocomplexes bound to agarose beads were isolated and washed thoroughly. The proteins were separated by SDS-PAGE (5–15%) and probed with antibodies against the indicated proteins. A single membrane was used, which was sequentially probed with the indicated antibodies after washing. Protein detection was carried out using ECL reagents. The results are representative of four experiments. IP, immunoprecipitation.