FIGURE 3.

Inhibition of PKC arrests vesicular movement of apoE-containing vesicles. HMDMs were transiently transfected with apoE-GFP cDNA and cultured for 24 h prior to performing microscopy in RPMI containing 0.1% BSA. Individual cells expressing apoE-GFP were identified using confocal Leica SP5 microscope on a heated stage, and GFP-positive vesicles were tracked and average speed was determined after exposure to CalpC (200 nm) or Ro-31-8220 (5 μm) for 10–20 min prior to imaging. A and B show a representative sequence of still images tracking apoE-GFP vesicles of the same cell before (A) and after (B) the addition of CalpC. Average speed (C) of vesicles was obtained from 100 representative vesicles from at least two independent macrophage preparations from different donors. Statistical analysis was performed using the Mann-Whitney U test with p < 0.0001 relative to control.