FIGURE 13.

At early time points endocytosed GPR17 accumulates in Rab5- or Rab4-positive vesicles. Oli-neu cells transiently transfected with cDNA encoding for GFP-Rab5a (Rab5) or GFP-Rab4a (Rab4) were labeled with anti-Nt-GPR17 antibodies and then stimulated with LTD₄ or UDP-glucose (UDPglc) for 15 min. After glycine washing, the cells were fixed, and the endocytosed GPR17 was visualized by labeling with Cy3-conjugated antibodies (red). The cells were then examined using an Axiovert 200M confocal system equipped with a spinning disc. Note the co-localization of Rab proteins with GPR17 (arrows) in small vesicles scattered throughout the cytoplasm. The images are representatives from three independent experiments. Bar = 5 μm.