FIGURE 14.

At later time points endocytosed GPR17 accumulates in Rab4-positive structures distributed in the perinuclear region. Oli-neu cells transiently transfected with cDNA encoding for GFP-Rab4a (Rab4) were labeled with anti-Nt-GPR17 antibodies and then stimulated with LTD₄ or UDP-glucose (UDPglc) for 30 min. After glycine washing, cells were fixed, and the endocytosed GPR17 was visualized by labeling with Cy3-conjugated antibodies (GPR). The cells were examined using an Axiovert 200M confocal system equipped with a spinning disc. Note the co-localization of GPR17 with Rab4 (arrows) in dot-like structures distributed in the perinuclear region. The images are representatives from three independent experiments. Bar = 5 μm.