FIGURE 15.
GPR17 exhibited a very minor degree of localization in Rab11-positive compartments. Oli-neu cells transiently transfected with cDNA encoding for GFP-Rab11a (Rab11) were labeled with anti-Nt-GPR17 antibodies and then stimulated with LTD4 or UDP-glucose (UDPglc) for 30 min. After glycine washing, the cells were fixed, and the endocytosed GPR17 was visualized by labeling with Cy3-conjugated antibodies (red, GPR). The cells were examined using an Axiovert 200M confocal system equipped with a spinning disc. The merged and single channel images shown are representatives from three independent experiments. Bar = 5 μm.