FIGURE 4.

Effect of scube1 knockdown and scube1 mRNA rescue on the production of erythrocytes. A–D, detection of hemoglobin in erythrocytes stained with ο-dianisidine in control MO or tMO1-injected embryos at 48 hpf. E–H, forced expression of scube1 mRNA alone had little phenotypic effect (G and H) but could partially rescue the anemic defect in the scube1 tMO1-injected embryos (E and F). The proportion of erythrocytes was restored by co-injection of 50 pg of scube1 mRNA in scube1 morphants at 48 hpf. Embryos are shown in lateral (A, C, E, and G) and ventral view (B, D, F, and H). The numbers indicated in the bottom left-hand corners of photographs are the number of affected embryos with phenotype similar to what is shown in the picture (left) and the total number of observed embryos (right).