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. 2012 Jan 6;39(2):396–406. doi: 10.1093/schbul/sbr177

Fig. 2.

Fig. 2.

NPAS3 protein expression in the dorsolateral prefrontal cortex across development. (A) Representative Western blots of the developmental cohort probed with an antibody to the NPAS3 protein (Abcam; Cat#ab59190), which can detect the predicted 100 kDa protein as well as a developmental age-specific protein migrating at 90 kDa. This 90 kDa NPAS3 immunoreactive protein was only observed in infant and toddler cases. β-actin was probed as a loading control and an internal control was used for normalization between Western blots. IC = internal control, N = neonate, I = infant, Tod = toddler, SA = school age, Teen = teenage, YA = young adult, A = adult. (B) The 100-kDa NPAS3 protein and β-actin were quantified by densitometry. NPAS3 expression was normalized to β-actin and presented as mean + SEM. *P < .05, **P < .01, ***P < .005. † 90 kDa NPAS3 immunoreactive protein band.