Figure 3.

Effect of MTE on HUVEC cell cycle progression. (A) The cells were treated with 0, 2.5, 5 and 7.5 mg/ml of MTE for 24 h, stained with PI and analyzed by FACS. The proportion of cells in each phase of the cell cycle was calculated using ModfitLT Version 3.0 Software. Representative assays are shown for each concentration of MTE. (B) The percentages of cells in the S phase, following the treatment with 0, 2.5, 5 and 7.5 mg/ml of MTE were compared. Data shown are the mean ± SD (error bars) from three independent experiments. ^*P<0.01, vs. control cells. MTE, Marsdenia tenacissima extract; HUVEC, human umbilical vein endothelial cell; PI, propidium iodide.