Figure 6.

(A) Effect of MTE on the mRNA expression levels of VEGF-A and VEGFR-2. Cells were treated with various concentrations of MTE for 24 h. The mRNA levels of VEGF-A and VEGFR-2 were determined by RT-PCR in HUVECs and HepG2s. GAPDH was used as an internal control. Data are representative of three independent experiments. (B) Effect of MTE on the protein expression levels of VEGF-A and VEGFR-2.(Bi) The protein levels of VEGF-A secreted in cell culture medium were determined by ELISA after HepG2 cells were treated with indicated concentrations of MTE for 24 h. (Bii) The protein levels of VEGF-A secreted in cell culture medium were determined by ELISA after HUVECs cells were treated with indicated concentrations of MTE for 24 h.. (Biii) The protein levels of VEGFR-2 in cell lysates were determined by ELISA after HUVECs cells were treated with indicated concentrations of MTE for 24 h. Data are the mean ± SD (error bars) from at least three independent experiments. ^*P<0.01, vs. control cells. MTE, Marsdenia tenacissima extract; VEGF-A, vascular endothelial growth factor-A; VEGFR-2, vascular endothelial growth factor receptor-2; HUVEC, human umbilical vein endothelial cell; HepG2, human hepatoma cell.