Figure 3.

Cell apoptosis under normal culture conditions in Saos-2 and MG-63 cells. Saos-2 (A) and MG-63 (B) cells were placed under normal culture conditions for 8 h. The percentage of terminal deoxynucleotidyl transferase mediated nick-end labeling (TUNEL)-positive cells in total cells after 8 h was used to determine the cell apoptosis rate. TUNEL assays were performed in normal control cells (NC), cells stably transfected with empty pcDNA3 vector (VC) and cells stably transfected with a pcDNA3-TWIST expression vector (TWIST) with or without LY294002 (LY; 5 μM) treatment in Saos-2 cells. TUNEL assays in NC, cells stably transduced with scrambled control short hairpin RNA (shRNA) (SC) and cells stably transduced with TWIST-shRNA (T-shRNA) with or without LY (50 μM) treatment in MG-63 cells. The cell apoptosis level is presented as the fold change relative to that of the NC (designated as 1).