Skip to main content
. 2013 Jan 7;5(3):857–861. doi: 10.3892/ol.2013.1111

Figure 5.

Figure 5

Western blot analysis of the phosphorylated Akt (P-Akt) level in Saos-2 and MG-63 cells. (A) The level of total Akt and P-Akt at serine 473 (ser473) in normal control cells (NC), cells stably transfected with empty pcDNA3 vector (VC), and cells stably transfected with pcDNA3-TWIST expression vector (TWIST) with or without LY294002 (LY; 50 μM) treatment was analyzed with western blot analysis in Saos-2 cells. (B) The level of total Akt and P-Akt at ser473 in NC, cells stably transduced with scrambled control short hairpin RNA (shRNA) (SC) and cells stably transduced with TWIST-shRNA (T-shRNA) with or without LY (50 μM) treatment was analyzed with western blot analysis in MG-63 cells. GAPDH blotting was used as a loading control. P-Akt (ser473), total Akt and GAPDH blots were measured by densitometry. The density of the P-Akt (ser473) blot was normalized against that of total Akt and GAPDH to obtain a relative P-Akt (ser473) blot density, which was expressed as the fold change compared with the relative P-Akt (ser473) blot density of NC (designated as 1). aP<0.05 compared with NC and VC (B) or SC (C); bP<0.05 compared with TWIST (B) or T-shRNA (C).